Journal: Journal of Experimental Pharmacology
Article Title: Antihypertensive Effects of Diosmetin in Hypertension-Induced Cardiovascular Abnormalities in Rats
doi: 10.2147/JEP.S541405
Figure Lengend Snippet: Representative Western blot images of membranes detected with chemiluminescence ( A ). Effects of diosmetin or telmisartan on AT 1 R ( B ), TGF-β ( C ), and MasR ( D ) protein expression in cardiac tissue collected from 2K-1C hypertensive rats. The data are expressed as mean ± SEMs. 2K-1C, two-kidney, one clip; DT20, diosmetin (20 mg/kg); DT40, diosmetin (40 mg/kg); T5, telmisartan (5 mg/kg). * p < 0.05 versus Sham + Vehicle, # p < 0.05 versus 2K-1C + Vehicle, † p < 0.05 versus 2K-1C + DT20.
Article Snippet: The membranes were blocked with 5% bovine serum albumin at room temperature (25°C) for 2 h before overnight incubation with primary antibodies against AT1R (sc-515884, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), MasR (sc-390453, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and TGF-β (sc-52893, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 4°C.
Techniques: Western Blot, Expressing